Selective Medium for Mosquito-Pathogenic Strains of Bacillus sphaericus
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چکیده
منابع مشابه
Conjugation by mosquito pathogenic strains of Bacillus sphaericus.
A mosquito pathogenic strain of Bacillus sphaericus carried out the conjugal transfer of plasmid pAM beta 1 to other strains of its own and two other serotypes. However, it was unable to conjugate with mosquito pathogens from three other serotypes, with B. sphaericus of other DNA homology groups or with three other species of Bacillus. Conjugation frequency was highest with a strain having an a...
متن کاملPhylogenetic analysis of Bacillus sphaericus and development of an oligonucleotide probe specific for mosquito-pathogenic strains.
The 16S rRNA gene sequences for six strains of Bacillus sphaericus representing five distinct DNA homology groups and one strain of Bacillus fusiformis have been determined. An-oligonucleotide probe based on the area approximately between position 186 and 198 (Escherichia coli numbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, hybridised to DNA from strains of DNA homology g...
متن کاملToxic activity of Bacillus sphaericus SSII-1 for mosquito larvae.
Using larvae of the mosquito Culex pipiens var. quinquefasciatus as a bioassay system, we have verified an earlier proposal that pathogenicity of Bacillus sphaericus SSII-1 is a toxin-mediated rather than an infectious process. Chloroform or ultraviolet-light treatments that decreased the viable count of SSII-1 cells by 4 or 5 logs did not significantly alter the ability of the bacterial cells ...
متن کاملLocalization of a mosquito-larval toxin of Bacillus sphaericus 1593.
Cell-free wall, membrane, and cytoplasmic fractions were prepared from Bacillus sphaericus 1593, which exhibited toxic activity against larvae of the mosquito Culex pipiens var. quinquefasciatus. Breakage of 12- to 14-h cells by sonication or French pressure cell yielded toxic material which could be assayed in a standard mosquito larva bioassay. When sporulating cells of strain 1593 were fract...
متن کاملSelective medium for Bacillus anthracis.
A need has existed for a selective medium that allows for growth of Bacillus anthracis while inhibiting common contaminants and closely related sporeformers (e.g., B. cereus). A selective medium (PLET medium) was developed containing the following ingredients added to Difco Heart Infusion Agar (HIA): polymyxin, 30 units/ml; lysozyme, 40 ,ug/ml; disodium ethylenediaminetetraacetate (EDTA), 300 ,...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1985
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.49.6.1532-1533.1985